Cryostats, the Bright OTF5000 Floor Standing Basic, LS and HS Models

Overview

The Bright OTF5000 floor standing biological cryostat is a medical device used to slice tissue and other samples very finely for analysis. The instrument is essentially a stationary freezer (the cryostat) capable of reaching temperatures within the range of −20 to −45 °C, with a rotary microtome incorporated into it that cuts histological and other samples extremely finely.

The microtome operates by use of an external wheel or lever which turns the blade and slices the sample. The Bright OTF5000 is suitable for an endless range of applications and is capable of cutting a wide diversity of specimens from undecalcified bone to brain, resins, plastics and plant tissue as well as more usual soft tissues.

The Bright OTF5000 cryostat range is suitable for both research and clinical operation and can be used with either solid knives or disposable blades in the microtome. The OTF5000 range is available in three standard models, with a long list of options and accessories to accommodate every sample slicing need in a laboratory.

All non-urgent functions are on a side panel, away from the essential systems..

Allowing full anti-roll plate adjustment for perfect results coupled with long lasting temperature stability, the OTF5000 is packed with features. Inquire about low cabinet height option, glove port, thin section conversions, large area sectioning and uv decontamination kits. A variety of holders, knives, honing supplies and lubricants are available.

All OTF5000 Cryostats come with Easi-set anti-roll plates (37mm, 50mm and 75mm), circular object holders (2 × 22mm, 1 × 37mm), 200mL bottle of low temperature oil, set of Allen keys, 113mL bottle of Cro-M-Bed, debris brush and debris tray.

The Bright 2212 Starlet compact bench top portable cryostat with radial, flat cutting rotary microtome is also available from Rose Scientific for use in laboratories where space is at a premium.

Features of the Bright OTF5000 Cryostats:

Three models, Basic, LE and LS
Uses solid knives or disposable blades with lever release disposable blade holder
Automatic evaporator defrost supplied as standard
The OTF5000 Basic includes Quick Freezer, Internal shelves, Evaporator defrost, Manual cutting system, 5040 microtome with fixed head, Heated window surround, Window demist system, Analog defrost clock, Side mounted non urgent controls and a full set of accessories
The OTF5000 LS specification adds 2 speed motorized advance and rewind, Fine object orientation and Cryomatic specimen temperature control
The OTF5000 HS specification adds to the LS features a 6 digit section counter, Dual refrigeration and Motor drive
All models are supplied with three anti-roll plates, two circular object holders, bottle of low temperature oil, Allen keys, bottle of Cro-M-Bed, debris brush and debris tray

Specifications (Click here):

Cryostats, the Bright OTF5000 Floor Standing Basic, LS and HS Models

Specifications

Model:	OTF5000 Basic	OTF5000 LS	OTF5000 HS
Lowest set temperature, single compressor: (°C)	-35	-35	-35
Dual refrigeration, twin compressors, automatic fail safe: (°C)	-	-	-40
Motor drive, maximum cutting speed: (mm/s)	-	-	35
Quick freezer temperature: (°C)	9 position, running at 10 below chamber, ultra low –80 version can be factory fitted at extra cost	9 position, running at 10 below chamber, ultra low –80 version can be factory fitted at extra cost	9 position, running at 10 below chamber, ultra low version cannot be fitted
Shelves:	3 internal, 1 external	3 internal, 1 external	3 internal, 1 external
Evaporator defrost:	Automatic with analog clock	Automatic with analog clock	Automatic with analog clock
Cutting stroke: (mm)	Manual with balanced handwheel	Manual with balanced handwheel	Manual with balanced handwheel
Cutting system:	5040 rotary microtome with beryllium hinges	5040 rotary microtome with beryllium hinges	5040 rotary microtome with beryllium hinges
Retraction on return stroke: (µm)	50	50	50
Object orientation: (°)	-	Fine, ± 8 in horizontal and vertical axes, 360 rotation	Fine, ± 8 in horizontal and vertical axes, 360 rotation
Motorized advance and rewind, 2 speed, autoreset: (µm/s)	-	116 and 164	116 and 164
Specimen temperature control:( °C)	-	-5 to minimum chamber temperature	-5 to minimum chamber temperature
Knife angle adjustment:(°)	25	25	25
Section thickness range, standard: (µm)	0.5 to 30 in 0.5 increments	0.5 to 30 in 0.5 increments	0.5 to 30 in 0.5 increments
Maximum head advance: (mm)	5.6	5.6	5.6
Maximum knife block adjustment, coarse control: (mm)	44	44	44
Normal working height: (mm)	990	990	990
Low working height: (mm)	890	890	890
Weight: (kg)	120	120	140
Dimensions with handle, low height model: (mm)	755×935×1200	755×935×1200	840×935×1200
Dimensions with handle, standard height model: (mm)	755×935×1300	755×935×1300	840×935×1300

Number	Description	Price $
QTF5000-002BASIC	OTF5000 Basic Cryostat fitted with standard knife holder assembly, Anti-roll system, 50230 solid microtome knife (22°angle), 110/115V, 50/60Hz	33,005.70
QTF5000-004BASIC	OTF5000 Basic Cryostat fitted with Quick Release Disposable Blade Holder, Anti-roll plate, Ten disposable blades, 110/115V, 50/60Hz	34,002.35
QTF5000-002LS	OTF5000-LS Cryostat fitted with standard knife holder assembly, Anti-roll system, 5023 solid microtome knife (22°angle), Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, 110/115V, 50/60Hz	35,760.40
OTF5000-004/LS	OTF5000-LS Cryostat fitted with Quick Release Disposable Blade Holder, complete with anti-roll plate and ten feather blades, Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, 110/115V, 50/60Hz	37,054.15
OTF5000-002HS	OTF5000-HS Cryostat fitted with standard knife holder assembly, Anti-roll system, 50230 solid microtome knife (22°angle), Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, Section counter (6-digit LCD display), Dual refrigeration -40°C chamber temperature, Motor drive sectioning and footswitch, 110/115V, 50/60Hz	44,863.30
OTF5000-004HS	OTF5000-HS Cryostat fitted with Quick Release Disposable Blade Holder, Anti-roll plate and ten feather blades, Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, Section counter (6-digit LCD display), Dual refrigeration -40°C chamber temperature, Motor drive sectioning and footswitch, 110/115V, 50/60Hz	45,833.75
53581	Bright Cryo-m-Bed, 113 mL bottle, blend of polyviol alcohol 217, thymol and water, for imbedding many different types of tissue over 18ºC to –20ºC and wider temperature range, pkg/6	430.10
57713	Bright Cryospray 134, 340g (300 mL) cans, aerosol freezing agent, 80 – 100% Tetrafluoroethane, 155 mm nozzle delivers jet at - 52°C, pkg/12 (not available because of shipping issues, inquire about alternatives)	Inquire
50539	Orientating object holder, with fine adjustment screws, factory fitted	1,501.90
50657	Orientating object holder, without fine adjustment screws, factory fitted	3,474.15
51637	Quick release hardboard holder, without orientation, for 5040-02	3,356.86
51637-1	Quick release hardboard holder, with orientation, for 5040-01	Inquire
50533	Quick release wax cassette holder, without orientation, for 5040-02	3,356.85
50209	Triangular glass knife holder, fixed	944.15
50681	Triangular glass knife holder, orientating. These accept 25mm high Latta-Hartmann knives with edges up to 15mm	2,065.40
52985	CO2 (gas) object holder and knife freezer	3,474.15
50208	Sloped (20º) knife holder	5,761.50
50213	Ralph knife holder	1,644.50
50214	Ralph knife holder with section float-out trough. For long edged glass knives with edges up to 38mm	1,751.45
52987	Illuminated magnifier	3,474.15
50230	Standard knife with box, C-profile, 189 x 27 x 10mm. 22º angle, tapped to accept holder for Shandon knife sharpener, safety cut-outs at each end, for 5030 and 5040 microtomes	882.05
50724	Knife sharpening back for 50230	313.95
50232-1	Hardened steel knife with box, C-profile, 160 x 35 x 9mm. 15º angle, suitable for large area sections	3,474.15
50210	Knife sharpening back for 50232-1	378.35
50207	Hardened steel knife with box, C-profile, 160 x 32 x 13mm. 23º, similar to 50232-1 but slightly heavier for tougher specimens	1,143.10
50211	Knife sharpening back for 50207	325.45
51675	Handle for 50230, 50232-1 and 50207	190.90
50234	Tungsten carbide tipped knife with box, 228 x 38 x 6mm. Suitable for cutting bone and hard plastics. needs to be resharpened at factory	2,970.45
52647	Stem for 50232-1 and 50234 knives.	146.05
52671	Knife sharpening kit. Includes hone, strop, oil, three grades of abrasive and instructions	1,367.35
57612	Coarse abrasive, 6g	75.90
57611	Fine abrasive, 6g	75.90
57610	Honing compound, 6g	75.90
50239	Clock oil, 6g	21.85
50300-1	Magnaplate non-adjustable anti-roll plate. Fits most knives and blades. picture shows sitting on a standard 50230 knife, package of 3 with a single removable handle	143.44
50140	Magnacut disposable blade system, with magnetic blade holder, for direct replacement of a solid knife system in a 5030 or 5040 microtome, includes 50 mm anti-roll plate, 10 blades, blade removal tool, micro-adjusting T-piece and stem, box and instructions	2,656.50
50133	Magnacut disposable blade system, with magnetic blade holder, for use without a fixed anti-roll plate, fits most cryostats, includes Magnaplate non-adjustable anti-roll plate, 10 blades, blade removal tool, box and instructions, picture is with Magnacut holder, disposable blade and Magnaplate non-adjustable anti-roll plate	2,544.95
50540	Magnacut disposable blade holder only	2,204.55
52727	Long-life disposable blades for Magnacut, C-profile, pkg/50	509.10
51564	Magnacut blade removing tool	37.95
50240	Feather blade holder with box	317.40
57903	Lever release Feather blade holder, accepts low profile S35 blades or similar, fits standard knife holders on many microtomes	Inquire
50241	Feather disposable blades, pack of 50	509.10
50260	5 - 300μm feed screw for non /Mar	3,069.35
50259	3 - 180μm feed screw for non /Mar	1,838.85
50258	2 - 120μm feed screw for non /Mar	1,675.55
50257	1 - 60μm feed screw for non /Mar	1,151.15
50255	0.5 - 30μm feed screw, supplied as standard for non /Mar	1,045.35
50256	0.2 - 12μm feed screw for non /Mar	1,386.90
50259-1	3 - 180μm feed screw for /Mar option	2,157.40
50258-1	2 - 120μm feed screw for /Mar option	1,997.55
50257-1	1 - 60μm feed screw for /Mar option	1,905.55
50255-1	0.5 - 30μm feed screw for Mar option, supplied as standard	1,367.35
50256-1	0.2 - 12μm feed screw for /Mar option	1,706.60
50727	Standard tubular holder, 22mm diameter, fits Bright 5030 microtome only	84.15
50734-1	Object holder, 22mm diameter, solid, suitable for most applications	133.40
50734-2	Object holder, 22mm diameter, solid, as 50734-1 but long type, gives greater clearance between specimen clamp and knife holder, ideal when multi-position glass slides are used	146.05
50735-1	Object holder, 37mm diameter, solid, suitable for larger specimens	292.10
50735-2	Object holder, 37mm diameter, solid, as 50735-1 but long type, suitable for larger specimens and gives extra clearance	305.90
50744	Object holder, 5mm diameter, solid, suitable for small specimens, particularly when orientation is required	133.40
50721	Object holder with stem, 50mm diameter, suitable for very large specimens	323.15
50741	Object holder with stem, 50 x 50mm square, suitable for very large specimens	374.90
50743	Object holder, 50 x 70mm	384.45
50221	Quick release object holder clamp, complete with 50 metal object discs	3,576.50
50203	Quick release object holder, fits into a standard vice clamp and takes 50690 metal discs	Inquire
50690	Metal object holder discs, 22mm, fits quick release object holder 50203, pkg/50	788.90
50204	Cork object holder discs, 22mm, pkg/100	152.95
50204-1	Cork object holder discs, 50 x 50mm square, pkg/50	215.05
50162	Adaptor, to accept Ames cryostat, saddle type object holders	870.55
52658	Vertical vice clamp	455.05
54791	Low temperature oil, 200ml bottle	123.05
54791-1	Low temperature oil, 4.54 litre drum	397.90
57355	Low temperature grease, 5g bottle	123.35
57344	Knife cleaning brush	258.75
57808	Anti static brush, 12mm	172.50
52531	Wax brush and debris tray	256.45
53573	Spare fuse set for 5040-001 or 5040-002 with 225MR	80.50
53574	Spare fuse set for 5040-001 or 5040-002 with 226MR	80.50
51695	Universal holder fits 5030 or 5040 microtomes that have fixed heads, can be fitted with object holders 50745, 50720, 50719, 50718 or 50712 to give coarse orientation	1,004.06
50745	Orienting object holder 5mm diameter, for small specimens	95.84
50720	Orienting object holder 22mm diameter, for medium size specimens	98.81
50719	Orienting object holder 37mm diameter, for larger specimens	149.81
50718	Orienting object holder 50mm diameter, for very large specimens	207.19
50712	Orienting object holder 50 × 50mm square, for very large specimens	216.75
50424	Easy-Set anti roll plate 37mm, side wings make it easy to adjust	95.63
50425	Easy-Set anti roll plate 50mm, side wings make it easy to adjust	98.81
50426	Easy-Set anti roll plate 75mm, side wings make it easy to adjust	105.19

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